DeLisa Research Group (DLRG)
We aim to engineer the protein machinery of simple bacteria for solving complex problems in biology and medicine
Synthetic Glycobiology
Immunoengineering
Proteostasis Engineering
We are currently pursuing research in the following areas:
Recent News
Selected Publications
Natarajan A, Jaroentomeechai T, Cabrera M, Mohammed J, Cox EC, Young O, Shajahan A, Vilkhovoy M, Vadhin S, Varner JD, Azadi P and DeLisa MP (2020) Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria. Nat Chem Biol 16: 1062–1070
Kightlinger W, Duncker KE, Ramesh A, Thames AN, Natarajan A, Lin L, Stark JC, Mrksich M, DeLisa MP and Jewett MC (2019) A cell-free biosynthesis platform for modular construction of protein glycosylation pathways. Nat Commun 10: 5404
Ludwicki, M.B., Li, J., Stephens, E.A., Roberts, R.W., Koide, S., Hammond, P.T. and DeLisa MP (2019) Broad-spectrum proteome editing with an engineered bacterial ubiquitin ligase mimic. ACS Cent Sci 5: 852-866
Meksiriporn B, Ludwicki MB, Stephens, EA, Jiang A, Lee H-C, Waraho-Zhmayev D, Kummer L, Brandl F, Plückthun A and DeLisa MP (2019) A survival-selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins. Nat Commun 10: 1830
Cox EC, Thornlow DN, Jones MA, Fuller J, Merritt JH, Paszek MJ, Alabi CA and DeLisa MP (2019) Antibody-mediated endocytosis of polysialic acid enables intracellular delivery and cytotoxicity of a glycan-directed antibody-drug conjugate. Cancer Res 79: 1810-1821
Kightlinger W, Lin L, Rosztoczy M, Li W, DeLisa MP, Mrksich M and Jewett MC (2018) Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases. Nat Chem Biol 14: 627-635
Stevenson T, Cywes-Bentley C, Moeller TD, Weyant KB, Putnam D, Chang Y-F, Jones BD, Pier GB and DeLisa MP (2018) Immunization with outer membrane vesicles displaying conserved surface polysaccharide antigen elicits broadly antimicrobial antibodies. Proc Natl Acad Sci USA 115: E3106-E3115
Mizrachi D, Robinson M-P, Ke N, Ren G, Berkmen M and DeLisa MP (2017) A water-soluble DsbB variant that catalyzes disulfide bond formation in vivo. Nat Chem Biol 13: 1022-1028
Chen L, Valentine JL, Huang C-J, Endicott CE, Moeller TD, Rasmussen JA, Fletcher JR, Boll J, Rosenthal JA, Dobruchowska J, Wang Z, Heiss C, Azalie P, Putnam D, Trent MS, Jones BD and DeLisa MP (2016) Outer membrane vesicles displaying engineered glycotopes elicit protective antibodies. Proc Natl Acad Sci USA 113: E3609-18
Robinson M-P, Ke N, Lobstein J, Peterson C, Szkodny A, Mansell TJ, Tuckey C, Riggs PD, Colossi PA, Noren CJ, Taron CH, DeLisa MP and Berkmen M (2015) Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6: 8072
Mizrachi D, Chen Y, Ke A, Pollack L, Turner RJ, Auchus RJ and DeLisa MP (2015) Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy. Nat Commun 6: 6826
Ollis AA, Zhang S, Fisher AC and DeLisa MP (2014) Engineered oligosaccharyltransferases with greatly relaxed acceptor site specificity. Nat Chem Biol 10: 816-22
Valderrama-Rincon J, Fisher AC, Merritt JH, Fan YY, Reading C, Chhiba KD, Aebi M and DeLisa MP (2012) An engineered eukaryotic glycosylation pathway in Escherichia coli. Nat Chem Biol 8: 434-6