DeLisa Research Group (DLRG)

We aim to engineer the protein machinery of simple bacteria for solving complex problems in biology and medicine

Synthetic Glycobiology
Proteostasis Engineering

The DLRG is currently focused on the following research themes:

Recent News

Selected Publications

Natarajan A, Jaroentomeechai T, Cabrera M, Mohammed J, Cox EC, Young O, Shajahan A, Vilkhovoy M, Vadhin S, Varner JD, Azadi P and DeLisa MP (2020) "Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria" Nature Chemical Biology 16: 1062–1070 Link


Kightlinger W, Duncker KE, Ramesh A, Thames AN, Natarajan A, Lin L, Stark JC, Mrksich M, DeLisa MP and Jewett MC (2019) "A cell-free biosynthesis platform for modular construction of protein glycosylation pathways" Nature Communications 10: 5404 Link

Ludwicki MB, Li J, Stephens EA, Roberts RW, Koide S, Hammond PT and DeLisa MP (2019) "Broad-spectrum proteome editing with an engineered bacterial ubiquitin ligase mimic" ACS Central Science 5: 852-866 Link

Meksiriporn B, Ludwicki MB, Stephens, EA, Jiang A, Lee H-C, Waraho-Zhmayev D, Kummer L, Brandl F, Plückthun A and DeLisa MP (2019) ​"A survival-selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins" Nature Communications 10: 1830 Link

Cox EC, Thornlow DN, Jones MA, Fuller J, Merritt JH, Paszek MJ, Alabi CA and DeLisa MP (2019) "Antibody-mediated endocytosis of  polysialic acid enables intracellular delivery and cytotoxicity of a glycan-directed antibody-drug conjugate" Cancer Research 79: 1810-1821 Link

Kightlinger W, Lin L, Rosztoczy M, Li W, DeLisa MP, Mrksich M and Jewett MC (2018) "Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases" Nature Chemical Biology 14: 627-635 Link

Stevenson T, Cywes-Bentley C, Moeller TD, Weyant KB, Putnam D, Chang Y-F, Jones BD, Pier GB and DeLisa MP (2018) "Immunization with outer membrane vesicles displaying conserved surface polysaccharide antigen elicits broadly antimicrobial antibodies" Proceedings of the National Academies of Sciences U S A 115: E3106-E3115​ Link

Mizrachi D, Robinson M-P, Ke N, Ren G, Berkmen M and DeLisa MP (2017) "A water-soluble DsbB variant that catalyzes disulfide bond formation in vivo" Nature Chemical Biology 13: 1022-1028  Link 

Chen L, Valentine JL, Huang C-J, Endicott CE, Moeller TD, Rasmussen JA, Fletcher JR, Boll J, Rosenthal JA, Dobruchowska J, Wang Z, Heiss C, Azalie P, Putnam D, Trent MS, Jones BD and DeLisa MP (2016) "Outer membrane vesicles displaying engineered glycotopes elicit protective antibodies" Proceedings of the National Academies of Sciences U S A 113: E3609-18 Link

Robinson M-P, Ke N, Lobstein J, Peterson C, Szkodny A, Mansell TJ, Tuckey C, Riggs PD, Colossi PA, Noren CJ, Taron CH, DeLisa MP and Berkmen M (2015) "Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria" Nature Communications 6: 8072 Link

Mizrachi D, Chen Y, Ke A, Pollack L, Turner RJ, Auchus RJ and DeLisa MP (2015) "Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy" Nature Communications 6: 6826 Link

Ollis AA, Zhang S, Fisher AC and DeLisa MP (2014) "Engineered oligosaccharyltransferases with greatly relaxed acceptor site specificity" Nature Chemical Biology 10: 816-22 Link

Valderrama-Rincon JD, Fisher AC, Merritt JH, Fan Y-Y, Reading CA, Chhiba K, Heiss C, Azadi P, Aebi M and DeLisa MP (2012) "An engineered eukaryotic glycosylation pathway in Escherichia coli" Nature Chemical Biology 8: 434-6 Link


Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University

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