135. High-resolution mapping of glycoprotein structure–activity relationships by shotgun scanning glycomutagenesis

Li M, Zheng X, Shanker S, Jaroentomeechai T, Koçer I, Byrne J, Cox EC, Labonte JW, Gray JJ and DeLisa MP

(in review) doi:

134. Structural basis for peptide substrate specificities of glycosyltransferase GalNAc-T2

Mahajan SP, Srinivasan Y, Labonte JW, Gray JJ and DeLisa MP

(in review) doi:

133. On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care

Stark JC, Jaroentomeechai T, Moeller TD, Dubner RS, Hsu KJ, Stevenson TC, DeLisa MP and Jewett MC

Sci Adv (in review) doi:

132. Effects of variable domain orientation on anti-HER2 single-chain variable antibody fragment expressed in the Escherichia coli cytoplasm

Koçer I, Cox E, DeLisa MP and Çelik E

Biotechnol Prog (in revision)

131. Two-tiered selection and screening strategy to increase functional enzyme production in Escherichia coli

Boock JT and DeLisa MP

Methods Mol Biol (in revision)

130. Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Hershewe JM, Warfel KF, Iyer SM, Peruzzi JA, Sullivan CJ, Roth EW, DeLisa MP, Kamat NP and Jewett MC

Nat Commun (in revision) doi:


129. Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli

Robinson M-P, Chang M, Cox EC, Li M, Jaroentomeechai T, Zheng X, Berkmen M and DeLisa MP

Nat Commun (in revision) doi:

128. Twin-arginine translocase component TatB performs folding quality control via a general chaperone activity

Taw MN, Boock JT, Rocco MA, Waraho-Zhmayev D and DeLisa MP

Nat Commun (in revision) doi:

127.  Cell-free synthetic glycobiology: designing and engineering glycomolecules outside the cell

Jaroentomeechai T, Taw MN, Li M, Aquino A, Agashe N, Chung S, Jewett MC and DeLisa MP

Frontiers in Chemistry 8: 645


126. Synthetic glycobiology: parts, systems, and applications

Kightlinger W, Warfel KF, DeLisa MP and Jewett MC

ACS Synth BIol 9: 1534-1562

125. Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria

Natarajan A, Jaroentomeechai T, Cabrera M, Mohammed J, Cox EC, Young O, Shajahan A, Vilkhovoy M, Vadhin S, Varner JD, Azadi P and DeLisa MP

Nat Chem Biol 16: 1062–1070

124. Induced fusion and aggregation of bacterial outer membrane vesicles: experimental and theoretical analysis 

Gnopo YMD, Misra A, Hsu H-L, DeLisa MP, Daniel S and Putnam D

J Colloid Interf Sci 578: 522-532

123. High-throughput synthesis and analysis of intact glycoproteins using SAMDI-MS

Techner J-M, Kightlinger W, Lin L, Hershewe JM, Ramesh A, DeLisa MP, Jewett MC and Mrksich M

Anal Chem 92: 1963-1971


122. Proteome editing using engineered proteins that hijack the ubiquitin proteasome pathway

Lopez-Barbosa N, Ludwicki MB and DeLisa MP

AIChE J 66: e16854 (special issue in honor of Frances Arnold)

121. A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

Kightlinger W, Duncker KE, Ramesh A, Thames AN, Natarajan A, Lin L, Stark JC, Mrksich M, DeLisa MP and Jewett MC

Nat Commun 10: 5404

120. Dual site-specific antibody conjugates for sequential and orthogonal cargo release

Thornlow DN, Cox EC, Walker JA, Sorkin M, Plesset JB, DeLisa MP and Alabi CA

Bioconjug Chem 30: 1702-1710

119. Broad-spectrum proteome editing with an engineered bacterial ubiquitin ligase mimic

Ludwicki MB, Li J, Stephens EA, Roberts RW, Koide S, Hammond PT and DeLisa MP

ACS Cent Sci 5: 852-866

118. A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins

Meksiriporn B, Ludwicki MB, Stephens, EA, Jiang A, Lee H-C, Waraho-Zhmayev D, Kummer L, Brandl F, Plückthun A and DeLisa MP

Nat Commun 10: 1830

117. Antibody-mediated endocytosis of polysialic acid enables intracellular delivery and cytotoxicity of a glycan-directed antibody-drug conjugate

Cox EC, Thornlow DN, Jones MA, Fuller J, Merritt JH, Paszek MJ, Alabi CA and DeLisa MP

Cancer Res 79: 1810-1821

116. Improving designer glycan production in Escherichia coli through model-guided metabolic engineering

Wayman J, Glasscock C, Mansell TJ, DeLisa MP and Varner JD
Metab Eng Commun 9: e00088

115. Glyco-recoded Escherichia coli: recombineering-based genome editing of native polysaccharide biosynthesis gene clusters

Yates LE, Natarajan A, Li M, Hale M, Mills DC and DeLisa MP

Metab Eng 53: 59-68


114. Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein

Mahajan SP, Meksiriporn B, Waraho-Zhmayev D, Weyant KB, Kocer I, Butler D, Messer A, Escobedo F and DeLisa MP

Sci Rep 8: 17611

113. The interplay of carbohydrate and carrier in antibacterial glycoconjugate vaccines

Moeller TD, Weyant KB and DeLisa MP

Adv Biochem Eng Biotechnol (in press)

112. Bacterial glycoengineering as a biosynthetic route to customized glycomolecules

Yates LE, Mills DC and DeLisa MP

Adv Biochem Eng Biotechnol (in press)​​

111. Metabolic engineering of glycoprotein synthesis in bacteria

Natarajan A, Jaroentomeechai T, Li M, Glasscock CJ and DeLisa MP

Emerg Top Life Sci 2: 419-432

110. Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Jaroentomeechai T, Stark JC, Natarajan A, Glasscock CJ, Yates LE, Hsu KJ, Mrksich M, Jewett MC and DeLisa MP

Nat Commun 9: 2686

109. Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

Kightlinger W, Lin L, Rosztoczy M, DeLisa MP, Mrksich M and Jewett MC

Nat Chem Biol 14: 627-635

108. Michael L. Shuler Tribute

Bonassar LJ, DeLisa MP, Putnam DA et al.

Biotechnol Bioeng 115: 1642-1645.

107. A flow cytometric approach to engineering Escherichia coli for improved eukaryotic protein glycosylation

Glasscock CJ, Jaroentomeechai T, Yates LE, Wilson JD, Merritt JH, Lucks JB and DeLisa MP

Metab Eng 47: 488-495

106. Immunization with outer membrane vesicles displaying a conserved polysaccharide antigen generates broadly bactericidal antibodies

Stevenson TC, Cywes-Bentley C, Moeller TD, Weyant KB, Putnam D, Chang Y-F,  Jones BD, Pier GB and DeLisa MP

Proc Natl Acad Sci U S A 115: E3106-E3115

105. Bioengineering a new generation of carbohydrate-based vaccines

Weyant KB, Mills DC and DeLisa MP

Curr Opin Chem Eng 19: 77–85 

104. A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

Schoborg JA, Hershewe JM, Stark JC, Kightlinger WK, Kath JE, Jaroentomeechai T, Natarajan A, DeLisa MP and Jewett MC

Biotechnol Bioeng 115: 739-750

103. Design and functional characterization of synthetic E3 ubiquitin ligases for targeted protein depletion

Baltz MR, Stephens EA and DeLisa MP

Curr Proton Chem Biol 10: 72-90


102. A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors

Hamilton BS, Wilson JD, Shumakovich MA, Fisher AC, Brooks JC, Pontes A, Naran R, Heiss C, Gao C, Kardish R, Heimburg-Molinaro J, Azadi P, Cummings RD, Merritt JH and DeLisa MP 

Sci Rep 7: 15907

101. A pipeline for studying and engineering single-subunit oligosaccharyltransferases

Jaroentomeechai T, Zheng X, Hershewe J, Stark JC,  Jewett MC and DeLisa MP

Methods Enzymol 597: 55-81

100. A water-soluble DsbB variant that catalyzes disulfide bond formation in vivo

Mizrachi D, Robinson M-P, Ke N, Ren G, Berkmen M and DeLisa MP

Nat Chem Biol 13: 1022-1028 [see News and Views by Kalistyn Burley & Celia Goulding]

99. A single dose and long lasting vaccine against pandemic influenza through the controlled release of a heterospecies tandem M2 sequence         

embedded within detoxified bacterial outer membrane vesicles

Watkins HC, Pagan CL, Childs HR, Posada S, Chau A, Rios J, Guarino C, DeLisa MP, Whittaker GR and Putnam D

Vaccine 35: 5373-5380

98.  Designer outer membrane vesicles as immunomodulatory systems – reprogramming bacteria for vaccine delivery

Gnopo YMD, Watkins HC, Stevenson TC, DeLisa MP and Putnam D

Adv Drug Deliv Rev 114: 132-142

97.  An engineered survival-selection assay for extracellular protein expression uncovers hypersecretory phenotypes in Escherichia coli

Natarajan A, Haitjema CH, Lee R, Boock JT and DeLisa MP

ACS Synth Biol 6: 875-883

96.  Safe recombinant outer membrane vesicles that display M2e elicit heterologous influenza protection

Watkins HC, Rappazzo CG, Higgins JS, Sun X, Brock N, Chau A, Misra A, Cannizzo J, King MR, Maines TR, Leifer CA, Whittaker GR, DeLisa MP and Putnam D

Mol Ther 25: 989-1002

95.  Human recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in a glycoengineered Escherichia coli strain

Almeciga-Diaz CJ, Pimentel-Vera LN, Rodriguez-Lopez A, Espejo-Mojica A, Ramirez AM, Cardona C, Reyes LH, DeLisa MP and Sanchez OF

Mol Gen Metab 120: S20.


94.  Modeling and analysis of hormone and mitogenic signal integration in prostate cancer 

Rogers K, Wayman J, Tasseff R, Gee C, DeLisa MP and Varner JD

bioRxiv doi:

93.  A molecularly complete planar bacterial outer membrane platform

Hsia CY, Chen L, DeLisa MP, and Daniel S

Sci Rep 6: 32715

92.  Immunization with outer membrane vesicles displaying designer glycotopes yields class-switched, glycan-specific antibodies

Valentine JL, Chen L, Perregaux EC, Weyant KB, Rosenthal JA, Heiss C, Azadi P, Putnam D, Moe GR, Merritt JH and DeLisa MP

Cell Chem Biol 23: 655-65


91.  Outer membrane vesicles displaying engineered glycotopes elicit protective antibodies

Chen L, Valentine JL, Huang C-J, Endicott C, Moeller T, Rasmussen J, Fletcher J, Boll J, Rosenthal JA, Dobruchowska J, Wang Z, Heiss C, Azadi P, Putnam D, Trent MS, Jones BD and DeLisa MP

Proc Natl Acad Sci U S A 113: E3609-18


90.  Engineered protein machines: emergent tools for synthetic biology

Glasscock CJ, Lucks JB and DeLisa MP

Cell Chem Biol 23: 45–56


89.  Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice

Rappazzo CG, Watkins HC, Guarino C, Chau A, Lopez JL, DeLisa MP, Leifer CA, Whittaker G and Putnam D

Vaccine 34: 1252-8


88.  Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences 

Ollis AA, Chai Y, Natarajan A, Perregaux EC, Jaroentomeechai T, Guarino C, Smith J, Zhang S and DeLisa MP

Sci Rep 5: 15237

87.  GlycoSNAP: a high-throughput screening methodology for engineering designer glycosylation enzymes

Ollis AA, Chai Y and DeLisa MP

Methods Mol Biol 1321: 37-47


86.  Characterizing metal-dependent nucleases of CRISPR/Cas prokaryotic adaptive immunity systems 

Nam KH, DeLisa MP and Ke A

Methods Mol Biol 1311: 265-76

85.  Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria

Robinson M-P, Ke N, Lobstein J, Peterson C, Szkodny A, Mansell TJ, Tuckey C, Riggs PD, Colussi PA, Noren CJ, Taron CH, DeLisa MP and Berkmen M

Nat Commun 6: 8072

84.  Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy

Mizrachi D, Chen Y, Liu J, Peng H-M, Ke A, Pollack L, Turner RJ, Auchus RJ and DeLisa MP

Nat Commun 6: 6826


83.  Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells

Boock JT, King BC, Taw M, Conrado RJ, Siu K-H, Stark J, Walker LP, Gibson DM and DeLisa MP

J Mol Biol 427: 1451-63


82.  Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them

Boock JT, Waraho-Zhmayev D, Mizrachi D, and DeLisa MP

Methods Mol Biol 1258: 79-97


81.  Glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions

Celik E, Ollis AA, Lasanajak Y, Fisher AC, Gur G, Smith DF and DeLisa MP

Biotechnol J 10: 199-209 [see Commentary by Dr. Eric Shusta]


80.  An engineered genetic selection for ternary protein complexes inspired by a natural three-component hitchhiker mechanism

Lee H-C, Portnoff AD, Rocco MA and DeLisa MP

Sci Rep 4: 7570


79.  Mechanistic insight into Th1-biased immune responses to recombinant subunit vaccines delivered by probiotic bacteria-derived OMVs

Rosenthal JA, Huang C-J, Doody AM, Leung T, Mineta K, Feng DD, Wayne EC, Nishimura N, Leifer C, DeLisa MP, Mendez S and Putnam D

PLoS ONE 9: e112802


78.  Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection

Waraho-Zhmayev D, Meksiriporn B, Portnoff AD and DeLisa MP

Protein Eng Des Sel 27: 351-8


77.  Engineered oligosaccharyltransferases with greatly relaxed acceptor site specificity

Ollis AA, Zhang S, Fisher AC and DeLisa MP

Nat Chem Biol 10: 816-22


76.  Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions

Park M, Sun Q, Liu F, DeLisa MP and Chen W

PLoS ONE 9: e97103


75.  Microbial biosynthesis of designer outer membrane vesicles

Baker JL, Chen L, Rosenthal JA, Putnam D and DeLisa MP

Curr Opin Biotechnol 29: 76-84


74.  Ubiquibodies: synthetic E3 ubiquitin ligases endowed with unnatural substrate specificity for targeted protein silencing

Portnoff AD, Stephens EA, Varner JD and DeLisa MP

J Biol Chem 289: 7844-55 [featured in SciBX]


73.  Split Cre recombinase effectively monitors protein-protein interactions in living bacteria

O’Brien SP and DeLisa MP

Biotechnol J 9: 355-61


72.  Pathogen-like particles: biomimetic vaccine carriers engineered at the nanoscale

Rosenthal JA, Baker JL, Chen L, Putnam D and DeLisa MP

Curr Opin Biotechnol 28: 51–58


71.  Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis

Sun Q, Madan B, Tsai S-L, DeLisa MP and Chen W

Chem Commun 50: 1423-5


70.  Universal genetic assay for engineering extracellular protein expression

Haitjema CH, Boock JT, Natarajan A, Dominguez MA, Gardner JG, Keating DH, Withers ST and DeLisa MP

ACS Synth Biol 3: 74–82 [featured on the cover]


69.  Directed evolution of Mycobacterium tuberculosis β-lactamase reveals gatekeeper residue that controls drug resistance and catalytic efficiency 

Feiler C, Fisher AC, Marrichi MJ, Wright L, Schmidpeter PAM, Blankenfeldt W, Pavelka M and DeLisa MP

PLoS ONE 8: e73123


68.  Engineered genetic selection links in vivo protein folding and stability with asparagine-linked glycosylation

Mansell TJ, Guarino C and DeLisa MP

Biotechnol J 8: 1445-51 [see Commentary by Dr. Danielle Tullman-Ercek]


67.  Low-dose recombinant vaccine antigen delivery by engineered outer membrane vesicles

Rosenthal JA, Leung T, DeLisa MP and Putnam DA

Nano LIFE 3: 1342002


66.  Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation

Baker J, Celik E and DeLisa MP

Trends Biotechnol 31: 313-23


65.  Glycans-by-design: engineering bacteria for the biosynthesis of complex glycans and glycoconjugates

Merritt JH, Ollis AA, Fisher AC and DeLisa MP

Biotechnol Bioeng 110: 1550-64


64.  A microbial sensor for discovering structural probes of protein misfolding and aggregation

Waraho-Zhmayev D, Gkogka L, Yu TY and DeLisa MP

Prion 7: 151-6


63.  Double-stranded endonuclease activity in B. halodurans clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas2 protein 

Nam KH, Ding F, Haitjema CH, Huang Q, DeLisa MP and Ke A

J Biol Chem 287: 35943-52 [featured on the cover]


62.  Cas5d protein processes pre-crRNA and assembles into a Cascade-like interference complex in subtype I-C/Dvulg CRISPR-Cas system

Nam KH, Haitjema CH, Ding F, DeLisa MP and Ke A

Structure 20: 1574-84 [see Preview by Dr. Nicole LaRonde-LeBlanc and featured on the cover]


61.  Twin-arginine translocase mutations that suppress folding quality control and permit export of misfolded substrate proteins

Rocco MA, Waraho-Zhmayev D and DeLisa MP

Proc Natl Acad Sci U S A 109: 13392-7


60.  Functional reconstitution of a tunable E3-dependent sumoylation pathway in Escherichia coli

O’Brien SP and DeLisa MP

PLoS ONE 7: e38671


59.  An engineered eukaryotic glycosylation pathway in Escherichia coli

Valderrama-Rincon JD, Fisher AC, Merritt JH, Yao-Yun F, Reading C, Chhiba KD, Heiss C, Azadi P, Aebi M and DeLisa MP

Nat Chem Biol 8: 434-6 [see Nature Chemical Biology press release and recommended article on F1000Prime]


58.  A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

Guarino C and DeLisa MP

Glycobiology 22: 596-601 


57.  Engineering antibody fitness and function using membrane-anchored display of correctly folded proteins

Karlsson AJ, Lim HK, Xu H, Rocco MA, Bratkowski MA, Ke A and DeLisa MP

J Mol Biol 416: 94-107 [Featured on the cover]


56.  Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection

Waraho D and DeLisa MP

Methods Mol Biol 813: 125-43


55.  The ribosomal exit tunnel as a target for optimizing protein expression in Escherichia coli

Contreras Martinez L, Boock JT, Kostecki JS and DeLisa MP

Biotechnol J 7: 354-60 [see Commentary by Dr. Francois Baneyx]

54.  DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency

Conrado RJ, Wu GC, Boock JT, Xu H, Chen SY, Lebar T, Turnšek J, Tomšič N, Avbelj M, Gaber R, Koprivnjak T, Mori J, Glavnik V, Vovk I, Benčina M, Hodnik V, Anderluh G, Dueber JE, Jerala R and DeLisa MP

Nucleic Acids Res 40: 1879-1889


53.  LuxS co-expression enhances yield of recombinant proteins in E. coli in part through post-transcriptional control of GroEL

Tsao C-Y, Wang L, Hashimoto Y, Yi H, March J, DeLisa MP, Wood TK, Valdes JJ, and Bentley WE

Appl Environ Microbiol 77: 2141-52


52.  Chlamydia trachomatis secretion of an immunodominant hypothetical protein (CT795) into host cell cytoplasm

Qi M, Lei L, Gong S, Liu Q, DeLisa MP and Zhong G

J Bacteriol 193: 2498-509


51.  Envelope stress is a trigger of CRISPR-mediated gene silencing in prokaryotes

Perez-Rodriguez R, Haitjema CH, Huang Q, Nam KH, Bernardis S, Ke A and DeLisa MP

Mol Microbiol 79: 584-99 [see MicroCommentary by Dr. Tracy Raivio]


50.  Genetic selection of solubility-enhanced proteins using the twin-arginine translocation system

Fisher AC, Rocco MA and DeLisa MP

Methods Mol Biol 705: 53-67


49.  Production of secretory and extracellular N-linked glycoproteins in Escherichia coli

Fisher AC, Haitjema CH, Guarino C, Celik E, Endicott CE, Reading CA, Merritt JH, Ptak AC, Zhang S and DeLisa MP

Appl Environ Microbiol 77: 871-81


48.  Secretion of the chlamydial protease CPAF requires Sec-dependent pathway

Chen D, Lei L, Flores R, DeLisa MP, Romesberg FE and Zhong G

Microbiology 156: 3031-40


47.  A phage display system for N-linked glycoproteins

Celik E, Fisher AC, Guarino C, Mansell TJ and DeLisa MP

Protein Sci 19: 2006-13 [see Highlight in Protein Science]


46.  A rapid protein folding assay for the bacterial periplasm

Mansell TJ, Linderman SW, Fisher AC and DeLisa MP

Protein Sci 19: 1079-90


45.  Kinetics and reaction coordinates of the reassembly of protein fragments via forward flux sampling

Borrero EE, Contreras Martinez L, DeLisa MP and Escobedo FA

Biophys J 98: 1911-20 


44.  Visualizing interactions along the Escherichia coli twin-arginine translocation pathway using protein fragment complementation

Kostecki JS, Li H, Turner RJ and DeLisa MP

PLoS ONE 5: e9225


43.  Delivery of foreign antigens by engineered outer membrane vesicle vaccines

Chen D, Metzger S, Osterrieder N, DeLisa MP and Putnam D

Proc Natl Acad Sci U S A 107: 3099-104 [see features in Nat Rev Drug Discov and SciBX]


42.  Mining mammalian genomes for folding competent proteins using Tat-dependent genetic selection in Escherichia coli

Lim H-K, Mansell TJ, Linderman SW, Fisher AC, Dyson MR and DeLisa MP

Protein Sci 18: 2537-49


41.  Site-specific labeling of surface proteins on living cells using genetically encoded peptides that bind fluorescent nanoparticles

Rocco MA, Kim J-Y, Burns A, Kostecki JS, Doody A, Wiesner U and DeLisa MP

Bioconjug Chem 20: 1482–89


40.  Synthetic metabolic pipelines

DeLisa MP and Conrado RJ

Nat Biotechnol 27: 728-9


39.  Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism

Waraho D and DeLisa MP

Proc Natl Acad Sci U S A 106: 3692-7


38.  Discovery of amyloid-beta aggregation inhibitors using an engineered assay for protein folding and solubility

Lee LL, Ha H, Chang Y-T and DeLisa MP

Protein Sci 18: 277-286


37.  Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery

Fisher AC and DeLisa MP

J Mol Biol 385: 299-311


36.  Following the path of a twin-arginine precursor along the TatABC translocase of Escherichia coli

Panahandeh S, Moser M, Maurer C, DeLisa MP and Müller M

J Biol Chem 283: 33267-75 


35.  Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes across the inner membrane of bacteria

Marrichi MJ, Camacho L, Russell DG and DeLisa MP

J Biol Chem 283: 35223-35


34.  Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy

Conrado R, Varner JD and DeLisa MP

Curr Opin Biotechnol 19: 492-9


33.  Exploration of twin-arginine translocation for the expression and purification of correctly folded proteins in Escherichia coli

Fisher AC, Kim J-Y, Perez-Rodriguez R, Tullman-Ercek D, Fish W, Henderson LA and DeLisa MP

Microb Biotechnol 1: 403-15


32.  Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control

Fisher AC and DeLisa MP

PLoS ONE 3: e2351


31.  Engineered bacterial outer membrane vesicles with enhanced functionality

Kim J-Y, Doody A, Chen DJ, Cremona GH, Shuler ML, Putnam DA and DeLisa MP

J Mol Biol 380: 51-66


30.  First international conference on biomolecular engineering

DeLisa MP and Haugh J

Biotechnol Prog 24: 1


29.  Engineering the protein folding landscape in Gram-negative bacteria

Mansell TJ, Fisher AC and DeLisa MP

Curr Protein Pept Sci 9: 138-149


28.  In silico protein fragmentation reveals the importance of critical nuclei in domain reassembly

Contreras Martinez L, Borrero EE, Escobedo F and DeLisa MP

Biophys J 94: 1575-88


27.  Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability

Contreras Martinez L and DeLisa MP

J Mol Biol 372: 513-24

26.  Stochastic reaction-diffusion simulation of enzyme compartmentalization reveals improved catalytic efficiency for a synthetic metabolic pathway 

Conrado RJ, Mansell TJ, Varner JD and DeLisa MP

Metab Eng 9: 355-63 [Featured on the cover]

25.  Pair velocity correlations among swimming bacteria are determined by force-quadrupole hydrodynamic interactions

Liao Q, Subramanian G, DeLisa MP, Koch DL and Wu M

Phys Fluids 19: 061701

24.  An essential role for the DnaK molecular chaperone in stabilizing over expressed substrates of the bacterial twin-arginine translocation pathway 

Perez-Rodriguez R, Fisher AC, Perlmutter JD, Hicks MG, Chanal A, Santini C-L, Wu L-F, Palmer T and DeLisa MP

J Mol Biol 367: 715-30


23.  Export pathway selectivity of Escherichia coli twin-arginine translocation signal peptides

Tullman-Ercek D, DeLisa MP, Kawarasaki Y, Iranpour P, Ribnicky B, Palmer T and Georgiou G

J Biol Chem 282: 8309-16


22.  Collective bacterial dynamics revealed using a three-dimensional population-scale defocused particle tracking technique

Wu M, Roberts JW, Kim S, Koch DL and DeLisa MP

Appl Environ Microbiol 72: 4987-94

21.  A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis 

Diao J, Young L, Kim S, Fogarty EA, Heilman SM, Zhou P, Shuler ML, Wu M and DeLisa MP

Lab Chip 6: 381-388 

20.  Protein translocation through a tunnel induces changes in folding kinetics: a lattice model study

Contreras Martinez L, Martinez-Veracoechea F, Pohkarel P, Stroock AD, Escobedo F and DeLisa MP

Biotechnol Bioeng 94: 105-117

19.  Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway

Fisher AC, Kim W and DeLisa MP

Protein Sci 15: 449-458 [Recommended article on F1000Prime]


18.  Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli

Kim J-Y, Fogarty EA, Lu FJ, Zhu H, Wheelock GD, Henderson LA and DeLisa MP

Appl Environ Microbiol 71: 8451-8459

17.  Identification of a twin-arginine translocation system in Pseudomonas syringe pv. tomato DC3000 and its contribution to pathogenicity and fitness 

Bronstein PA, Marrichi MJ, Cartinhour S, Schneider DJ and DeLisa MP

J Bacteriol 187: 8450-8461 [Supplemental Data]


16.  Dissecting the twin-arginine translocation pathway using genome-wide analysis

Bronstein PA, Marrichi MJ and DeLisa MP

Res Microbiol 155: 803-810


15.  A little help from my friends: quality control of presecretory proteins in bacteria

Fisher AC and DeLisa MP

J Bacteriol 186: 7467-7473

14.  An engineered pathway for the formation of protein disulfide bonds

Masip L, Pan J, Haldar S, Penner-Han JE, DeLisa MP, Georgiou G, Bardwell J and Collet J-F

Science 303: 1185-1189 [Recommended article on F1000Prime]


13.  Phage shock protein PspA of Escherichia coli relieves the saturation of protein export via the Tat pathway

DeLisa MP, Lee P, Palmer T and Georgiou G

J Bacteriol 186: 366-373 [Recommended article on F1000Prime]


12.  Folding quality control in the export of proteins via the bacterial twin-arginine translocation pathway

DeLisa MP, Tullman D and Georgiou G

Proc Natl Acad Sci U S A 100: 6115-6120 [Recommended article on F1000Prime]


11.  Genetic analysis of the twin-arginine translocator pathway in bacteria

DeLisa MP, Samuelson P, Palmer T and Georgiou G

J Biol Chem 277: 29825-29831


10.  Bacterial autoinduction: looking outside the cell for new metabolic engineering targets

DeLisa MP and Bentley WE

Microb Cell Fact 1: 5

9.    Quorum signaling via AI-2 communicates the "metabolic burden" associated with heterologous protein production in Escherichia coli

DeLisa MP, Valdes JJ and Bentley WE

Biotechnol Bioeng 75: 439-450

8.    DNA microarray-based identification of genes controlled by AI-2 stimulated quorum sensing in Escherichia coli

DeLisa MP, Wu C-F, Wang L, Valdes JJ and Bentely WE

J Bacteriol 183: 5239-5247


7.    Mapping stress-induced changes in autoinducer AI-2 production in chemostat-cultivated Escherichia coli

DeLisa MP, Valdes JJ and Bentley WE

J Bacteriol 183: 2918-2928

6.    Genomic analysis of high cell density recombinant Escherichia coli fermentation and cell “conditioning” for improved recombinant protein yield

Gill RT, DeLisa MP, Valdes JJ and Bentley WE

Biotechnol Bioeng 72: 85-95


5.    Generic model control of induced protein expression in high cell density cultivation of Escherichia coli using on-line GFP-fusion monitoring

DeLisa MP, Chae HJ, Weigand WA, Rao G, Valdes JJ and Bentley WE

Bioproc Biosyst Eng 24: 83-91


4.    Evanescent wave long period fiber Bragg grating as an immobilized antibody biosensor

DeLisa MP, Zhang Z, Shiloach M, Pilevar S, Davis CC, Sirkis S and Bentley WE

Anal Chem 72: 2895-2900 


3.    OmpT activity and expression increase in response to recombinant chloramphenicol acetyl-transferase overexpression

Gill RT, DeLisa MP, Shiloach M, Holoman TR and Bentley WE

J Mol Microbiol Biotechnol 2: 283-289


2.    Framework for on-line optimization of recombinant protein expression in high cell density Escherichia coli cultures using GFP-fusion monitoring

Chae HJ, DeLisa MP, Cha HJ, Weigand WA and Bentley WE

Biotechnol Bioeng 69: 275-285


1.    Monitoring fusion protein expression during high cell density cultivation of Escherichia coli using on-line green fluorescent protein sensor

DeLisa MP, Li J, Rao G, Weigand WA and Bentley WE

Biotechnol Bioeng 65: 54-64

Books and Book Chapters

5.    Bioprocess Engineering

Shuler ML, Kargi F and DeLisa MP

Third Edition. New Jersey: Prentice Hall


4.    Protein folding and solubility: pathways and high-throughput assays

Fisher AC, Mansell TJ and DeLisa MP

In: Lutz S and Bornscheuer U, editors. Protein Engineering Handbook. Germany: John Wiley and Sons, Inc.


3.    Expression engineering of synthetic antibodies using ribosome display

Contreras Martinez L and DeLisa MP

In: Dyson M and Durocher Y, editors. Expression Systems - Methods Express. Oxfordshire, UK: Scion Publishing


2.    Engineering multifunctional enzyme systems for optimized metabolite transfer between sequential conversion steps

Conrado RJ, Mansell TJ and DeLisa MP

In: Smolke CD, editor. Handbook for Metabolic Pathway Engineering. San Diego: CRC Press

1.    Mapping stresses in Escherichia coli to improve yield: Examining global gene regulation and “cell conditioning” strategies

DeLisa MP, Gill RT and Bentley WE

In: Merten O-W, editor. Recombinant Protein Production with Prokaryotic and Eukaryotic Cells A Comparative View on Host Physiology: Springer

Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University

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