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Publications

2024

167. Bidstrup E, Hill K, Chisti A, Aw R, Jewett MC, Kightlinger W and DeLisa MP

Glycosylation of structured protein domains in cell-free reaction environments

ACS Central Science  (in preparation)

166. Hong L, Ye T, Wang T, Zhao L, Vincoff S, Chen T, Pertsemlidis S, DeLisa MP and Chatterjee P

Programmable protein stabilization with language model-derived peptide guides

Nature Communications (in preparation)

165. Thaiprayoon A, Chantarsasorn Y, Oonanant W […] Loison F, Kruse AC, DeLisa MP and Waraho-Zhmayev D

 Isolation of PCSK9-specific nanobodies from synthetic libraries using a combined protein selection strategy

Scientific Reports (in review)

 

164. Kannan N, Choi A, Rivera De Jesus MA […] Curly SM, August A, DeLisa MP, Whittaker GR and Putnam D

Intranasal vaccination with recombinant TLR2-active outer membrane vesicles containing sequential M2e epitopes protects against lethal influenza A challenge

Vaccines (in review)

163. Sotomayor B, Donahue TC, Mahajan SP […] Azadi P, Gray JJ, Jewett MC, Wang L-X and DeLisa MP

Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length antibodies in Escherichia coli

Nature Chemical Biology (in review)

162. Chen T, Dumas M, Watson […] Kholina K, Goel S, DeLisa MP, Truant R, Aquilar-Carreno H and Chatterjee P

PepMLM: target sequence-conditioned generation of therapeutic peptide binders via span masked language modeling

Nature Biotechnology (in review).

161. DeWinter MA, Wong DA, Fernandez R, Kightlinger W, Thames AH, DeLisa MP and Jewett MC

Establishing a cell-free glycoprotein synthesis system for enzymatic N-GlcNAcylation

ACS Chemical Biology (in revision)

 

160. Pimentel-Vera LN, Rodríguez-López A, Ramírez A, [...] Jaroentomeechai T, DeLisa MP, Sánchez OF, and Alméciga-Díaz CJ

Novel human recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in a glyco-engineered Escherichia coli strain

Heliyon (in revision)

159. Bhat S, Palepu K, Hong L, Mao J, Ye T, Iyer R, Zhao L, [...] Desphande AJ, Soderling SH, DeLisa MP and Chatterjee P

Generation and prioritization of novel target-binding peptides from sequence alone with PepPrCLIP

Nature (in revision)

158. Kuo J,  Colville MJ,  Sorkin MR, Kuo JLK, Huang L-T, [...] Beacham GM, Hollopeter G, DeLisa MP, Alabi CA and Paszek MJ

Bio-orthogonal glycan imaging of culture cells and whole animal C. elegans with expansion microscopy

ACS Central Science (in revision) Link

 

157. Palma J, Bunyatov M, Hulbert SW, Jewett MC and DeLisa MP

Bacterial glycoengineering: cell-based and cell-free routes for producing biopharmaceuticals with customized glycosylation

Current Opinion in Chemical Biology (accepted)

*Special issue on “Chemical Glycobiology”

156. Alamgir A, Ghosal S, DeLisa MP and Alabi CA

Bioreversible anionic cloaking enables intracellular protein delivery with ionizable lipid nanoparticles

ACS Central Science (in press) Link

*Featured on supplementary cover

155. Park S, Colville MJ, Shurer CR, Kuo J, [...] DeLisa MP, Lammerding J, Zipfel WR, Fischbach C, Reesink HL and Paszek MJ

Immunoengineering can overcome the glycocalyx armor of cancer cells

Nature Materials 23: 429-438 Link

154. Weyant KB, Oloyede A and DeLisa MP

On-demand vaccine production via dock-and-display of biotinylated antigens on bacterial extracellular vesicles

Methods in Molecular Biology (in press)

153. Williams AJ, Wong DA, Desai P, Nguyen PM, Qin Y, Warfel KF, Jewett MC and DeLisa MP

Cell-free systems for the production of conjugate vaccines

Methods in Molecular Biology (in press)

152. Bidstrup E, Kwon YH, Kim K, Bandi CK, Aw R, Jewett MC and DeLisa MP

Cell-free systems for the production of glycoproteins

Methods in Molecular Biology 2762: 309-328 Link

151. Lee J-J, Abdullah M, Liu J, Carvalho IA, Junior AS, Moreira M, Mohammed H, DeLisa MP, McDonough SP and Chang Y-F

Proteomic profiling of membrane vesicles from Mycobacterium avium subsp. paratuberculosis:  Navigating towards an in silico design of a multi-epitope vaccine targeting membrane vesicle proteins

Journal of Proteomics 292: 105058 Link

2023

150. Brixi G, Ye T, Hong L, Wang T, Monticello C, Lopez-Barbosa N, Vincoff S, Yudistyra V [...] Petersen L, DeLisa MP and Chatterjee P

SaLT&PepPr is an interface-predicting language model for designing peptide-guided protein degraders

Communications Biology 6: 1081 Link

149. Hulbert SW, Desai P, Jewett MC, DeLisa MP and Williams AJ

Glycovaccinology: the design and engineering of carbohydrate-based vaccine components

Biotechnology Advances 68: 108234 Link

148. Robinson M-P, Jung J, Lopez-Barbosa N, Chang M, Li M, Jaroentomeechai T, Cox E, Zheng X, Berkmen M and DeLisa MP

Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli

Nature Communications 14: 3514 Link

147. Stark JC, Jaroentomeechai T, Warfel KF, Hershewe JM, DeLisa MP and Jewett MC

Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates

Nature Protocols 18: 2374-98 Link

146.  Moeller TD, Shah SB, Lai K, Lopez-Barbosa N, Desai P, Wang W, Zhong Z, Redmond D, Singh A and DeLisa MP

Profiling germinal center-like B cell responses to conjugate vaccines using synthetic immune organoids

ACS Central Science 9: 787-804 Link

*Featured in ACS Press Room "Testing vaccine candidates quickly with lab-grown mini-organs​"

145. Weyant KB, Oloyede A, Pal S, Liao J, Rivera-De Jesus M [...] Locher C, de la Maza LM, Coleman MA and DeLisa MP

A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens

Nature Communications 14: 464 Link

*Editor's Highlight - top 50 best papers in “Microbiology and infectious diseases”

144. Williams AJ, Warfel KF, Desai P, Li J, Lee JJ, Wong, DA, Nguyen PM, Qin Y, Sobol S, Jewett MC, Chang Y-F and DeLisa MP

A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice

Frontiers in Molecular Biosciences 10: 1085887 Link

 

143. Warfel KF, Williams AJ, Wong DA, Sobol SE, Desai P, Li J, Chang Y-F, DeLisa MP, Karim AS and Jewett MC

A low-cost, thermostable, cell-free protein synthesis platform for on-demand production of conjugate vaccines

ACS Synthetic Biology 12: 95-107 Link

2022

142. Chung SS, Bidstrup EJ, Hershewe JM, Warfel KF, Jewett MC and DeLisa, MP

Ribosome stalling of N-linked glycoproteins in cell-free extracts

ACS Synthetic Biology 11: 3892-3899 Link

141. Jaroentomeechai T, Kwon YH, Liu Y [...] Wilson JD, Li M, Chapla DG, Moremen KW, Jewett MC, Mizrachi D and DeLisa MP

A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans

Nature Communications 13: 6325 Link

*Editors’ Highlight - top 50 best papers in “Biotechnology and methods”

140. Taw MN, Boock JT, Sotomayor B, Kim D, Rocco MA, Waraho-Zhmayev D and DeLisa MP

Twin-arginine translocase component TatB performs folding quality control via a general chaperone activity

Scientific Reports 12: 14862 Link

139. Jahi F, Zaferani M, Lopez-Barbosa N, DeLisa MP and Abbaspourrad A

Sheathless inertial microfluidic cell separation via a serpentine–contraction–expansion device coupled with a combinatorial extraction regulator

Microfluidics and Nanofluidics 26: 54 Link

138. Clausen H, Wandall HH, DeLisa MP, Steentoft C, Stanley P, and Schnaar RL

Glycosylation engineering

In: Varki A et al., editors. Essentials of Glycobiology [Internet]. 4th edition. Cold Spring Harbor Laboratory Press Link

137. Boock JT and DeLisa MP

Two-tiered selection and screening strategy to increase functional enzyme production in Escherichia coli

Methods in Molecular Biology 2406: 169-187 Link

2021

136. Aquino AK, Manzer ZA, Daniel S and DeLisa MP

Glycosylation-on-a-chip: a flow-based microfluidic system for cell-free glycoprotein synthesis

Frontiers in Molecular Biosciences 8: 782905 Link

135. Taw MN, Li M, Kim D, Rocco MA, Waraho-Zhmayev D and DeLisa MP

Engineering a hyper-secreting strain of Escherichia coli by directed co-evolution of multiprotein translocation machinery

ACS Synthetic Biology 10: 2947-2958 Link

134. Li M, Zheng X, Shanker S, Jaroentomeechai T, Moeller TD, Hulbert S [...] Cox EC, Labonte JW, Gray JJ and DeLisa MP

Shotgun scanning glycomutagenesis: a simple and efficient strategy for constructing and characterizing neoglycoproteins

Proceedings of the National Academies of Sciences U S A 118: e2107440118 Link 

133. Stephens EA, Ludwicki MB, Meksiriporn B, Li M, Ye T, Monticello C [...] Kummer L, Zhou P, Plückthun A and DeLisa MP

Engineering single pan-specific ubiquibodies for targeted degradation of all forms of endogenous ERK protein kinase

ACS Synthetic Biology 10: 2396-2408 Link

132. Hershewe JM, Warfel KF, Iyer SM, Peruzzi JA, Sullivan CJ, Roth EW, DeLisa MP, Kamat NP and Jewett MC

Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Nature Communications 12: 2363 Link

131. Mahajan SP, Srinivasan Y, Labonte JW, DeLisa MP and Gray JJ

Structural basis for peptide substrate specificities of glycosyltransferase GalNAc-T2

ACS Catalysis 11: 2977-2991 Link

130. Stark JC, Jaroentomeechai T, Moeller TD, Hershewe JM, Warfel KF [...] Jones BD, DeLisa MP and Jewett MC

On-demand biomanufacturing of protective conjugate vaccines

Science Advances 7: eabe9444 Link

129. Koçer I, Cox E, DeLisa MP and Çelik E

Effects of variable domain orientation on anti-HER2 single-chain variable antibody fragment expressed in the Escherichia coli cytoplasm

Biotechnology Progress 37: e3102 Link

128. Moeller TD, Weyant KB and DeLisa MP

The interplay of carbohydrate and carrier in antibacterial glycoconjugate vaccines

Advances in Biochemical Engineering / Biotechnology 175: 355-378 Link

127. Yates LE, Mills DC and DeLisa MP

Bacterial glycoengineering as a biosynthetic route to customized glycomolecules

Advances in Biochemical Engineering / Biotechnology 175: 167-200 Link

2020

126. Han C, Xia K, Yang J, Zhang H, DeLisa MP and Liang X

Investigation of lipid profile in Acetobacter pasteurianus Ab3 against acetic acid stress during vinegar production

Extremophiles 24: 909-922 Link

125. Jaroentomeechai T, Taw MN, Li M, Aquino A, Agashe N, Chung S, Jewett MC and DeLisa MP

Cell-free synthetic glycobiology: designing and engineering glycomolecules outside the cell

Frontiers in Chemistry 8: 645 Link

124. Kightlinger W, Warfel KF, DeLisa MP and Jewett MC

Synthetic glycobiology: parts, systems, and applications

ACS Synthetic Biology 9: 1534-1562 Link

123. Natarajan A, Jaroentomeechai T, Cabrera M, Mohammed J, Cox EC, Young O [...] Varner JD, Azadi P and DeLisa MP

Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria

Nature Chemical Biology 16: 1062–1070 Link

 

122. Gnopo YMD, Misra A, Hsu H-L, DeLisa MP, Daniel S and Putnam D

Induced fusion and aggregation of bacterial outer membrane vesicles: experimental and theoretical analysis

Journal of Colloid and Interface Science 578: 522-532 Link

121. Techner J-M, Kightlinger W, Lin L, Hershewe JM, Ramesh A, DeLisa MP, Jewett MC and Mrksich M

High-throughput synthesis and analysis of intact glycoproteins using SAMDI-MS

Analytical Chemistry 92: 1963-1971 Link

​​2019

120. Lopez-Barbosa N, Ludwicki MB and DeLisa MP

Proteome editing using engineered proteins that hijack the ubiquitin proteasome pathway

AIChE Journal 66: e16854 Link

*Special issue in honor of Frances Arnold

​119. Kightlinger W, Duncker KE, Ramesh A, Thames AN, Natarajan A, Lin L, Stark JC, Mrksich M, DeLisa MP and Jewett MC

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

Nature Communications 10: 5404 Link

118. Thornlow DN, Cox EC, Walker JA, Sorkin M, Plesset JB, DeLisa MP and Alabi CA

Dual site-specific antibody conjugates for sequential and orthogonal cargo release

Bioconjugate Chemistry 30: 1702-1710 Link

117. Ludwicki MB, Li J, Stephens EA, Roberts RW, Koide S, Hammond PT and DeLisa MP

Broad-spectrum proteome editing with an engineered bacterial ubiquitin ligase mimic

ACS Central Science 5: 852-866 Link

116. Meksiriporn B, Ludwicki MB, Stephens, EA [...] Waraho-Zhmayev D, Kummer L, Brandl F, Plückthun A, and DeLisa MP

A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins

Nature Communications 10: 1830 Link

115. Cox EC, Thornlow DN, Jones MA, Fuller J, Merritt JH, Paszek MJ, Alabi CA and DeLisa MP

Antibody-mediated endocytosis of polysialic acid enables intracellular delivery and cytotoxicity of a glycan-directed antibody-drug conjugate

Cancer Research 79: 1810-1821 Link

114. Wayman J, Glasscock C, Mansell TJ, DeLisa MP and Varner JD

Improving designer glycan production in Escherichia coli through model-guided metabolic engineering

Metabolic Engineering Communications 9: e00088 Link
 

113. Yates LE, Natarajan A, Li M, Hale M, Mills DC and DeLisa MP

Glyco-recoded Escherichia coli: recombineering-based genome editing of native polysaccharide biosynthesis gene clusters

Metabolic Engineering 53: 59-68 Link

2018

112. Mahajan SP, Meksiriporn B, Waraho-Zhmayev D, Weyant KB, Kocer I, Butler D, Messer A, Escobedo F and DeLisa MP

Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein

Scientifiic Reports 8: 17611 Link

111. Natarajan A, Jaroentomeechai T, Li M, Glasscock CJ and DeLisa MP

Metabolic engineering of glycoprotein synthesis in bacteria

Emerging Topics in Life Sciences 2: 419-432 Link

110. Jaroentomeechai T, Stark JC, Natarajan A, Glasscock CJ, Yates LE, Hsu KJ, Mrksich M, Jewett MC and DeLisa MP

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Nature Communications 9: 2686 Link

109. Kightlinger W, Lin L, Rosztoczy M, DeLisa MP, Mrksich M and Jewett MC

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

Nature Chemical Biology 14: 627-635 Link

108. Bonassar LJ, DeLisa MP, Putnam DA et al.

Michael L. Shuler Tribute

Biotechnology and Bioengineering 115: 1642-1645 Link

107. Glasscock CJ, Jaroentomeechai T, Yates LE, Wilson JD, Merritt JH, Lucks JB and DeLisa MP

A flow cytometric approach to engineering Escherichia coli for improved eukaryotic protein glycosylation

Metabolic Engineering 47: 488-495 Link

106. Stevenson TC, Cywes-Bentley C, Moeller TD, Weyant KB, Putnam D, Chang Y-F,  Jones BD, Pier GB and DeLisa MP

Immunization with outer membrane vesicles displaying a conserved polysaccharide antigen generates broadly bactericidal antibodies

Proceedings of the National Academies of Sciences U S A 115: E3106-E3115 Link

​​

105. Weyant KB, Mills DC and DeLisa MP

Bioengineering a new generation of carbohydrate-based vaccines

Current Opinion in Chemical Engineering 19: 77–85 Link

104. Schoborg JA, Hershewe JM, Stark JC, Kightlinger WK [...] Jaroentomeechai T, Natarajan A, DeLisa MP and Jewett MC

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

Biotechnology and Bioengineering 115: 739-750 Link

103. Baltz MR, Stephens EA and DeLisa MP

Design and functional characterization of synthetic E3 ubiquitin ligases for targeted protein depletion

Current Protocols in Chemical Biology 10: 72-90 Link

 

 

2017​

102. Hamilton BS, Wilson JD, Shumakovich MA, Fisher AC, Brooks JC [...] Azadi P, Cummings RD, Merritt JH and DeLisa MP

A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors

Scientific Reports 7: 15907 Link

 ​

101. Jaroentomeechai T, Zheng X, Hershewe J, Stark JC,  Jewett MC and DeLisa MP

A pipeline for studying and engineering single-subunit oligosaccharyltransferases

Methods in Enzymology 597: 55-81 Link

100. Mizrachi D, Robinson M-P, Ke N, Ren G, Berkmen M and DeLisa MP

A water-soluble DsbB variant that catalyzes disulfide bond formation in vivo

Nature Chemical Biology 13: 1022-1028 Link

*Featured in News and Views by Kalistyn Burley & Celia Goulding

99. Watkins HC, Pagan CL, Childs HR, Posada S, Chau A, Rios J, Guarino C, DeLisa MP, Whittaker GR and Putnam D

A single dose and long lasting vaccine against pandemic influenza through the controlled release of a heterospecies tandem M2 sequence embedded within detoxified bacterial outer membrane vesicle

Vaccine 35: 5373-5380​ Link

98. Gnopo YMD, Watkins HC, Stevenson TC, DeLisa MP and Putnam D

Designer outer membrane vesicles as immunomodulatory systems – reprogramming bacteria for vaccine delivery

Advanced Drug Delivery Reviews 114: 132-142 Link

97. Natarajan A, Haitjema CH, Lee R, Boock JT and DeLisa MP

An engineered survival-selection assay for extracellular protein expression uncovers hypersecretory phenotypes in Escherichia coli

ACS Synthetic Biology 6: 875-883 Link

96. Watkins HC, Rappazzo CG, Higgins JS, Sun X [...] King MR, Maines TR, Leifer CA, Whittaker GR, DeLisa MP and Putnam D

Safe recombinant outer membrane vesicles that display M2e elicit heterologous influenza protection

Molecular Therapy 25: 989-1002 Link

95. Almeciga-Diaz CJ, Pimentel-Vera LN, Rodriguez-Lopez A [...] Cardona C, Reyes LH, DeLisa MP and Sanchez OF

Human recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in a glycoengineered Escherichia coli strain

Molecular Genetics and Metabolism 120: S20 Link

2016​

94. Rogers K, Wayman J, Tasseff R, Gee C, DeLisa MP and Varner JD

Modeling and analysis of hormone and mitogenic signal integration in prostate cancer

bioRxiv Link

93. Hsia CY, Chen L, DeLisa MP, and Daniel S

A molecularly complete planar bacterial outer membrane platform

Scientific Reports 6: 32715 Link

92.  Valentine JL, Chen L, Perregaux EC, Weyant KB [...] Heiss C, Azadi P, Putnam D, Moe GR, Merritt JH and DeLisa MP

Immunization with outer membrane vesicles displaying designer glycotopes yields class-switched, glycan-specific antibodies

Cell Chemical Biology 23: 655-65 Link

91. Chen L, Valentine JL, Huang C-J, Endicott C, Moeller T [...] Heiss C, Azadi P, Putnam D, Trent MS, Jones BD and DeLisa MP

Outer membrane vesicles displaying engineered glycotopes elicit protective antibodies

Proceedings of the National Academies of Sciences U S A 113: E3609-18 Link

90. Glasscock CJ, Lucks JB and DeLisa MP

Engineered protein machines: emergent tools for synthetic biology

Cell Chemical Biology 23: 45–56 Link

89. Rappazzo CG, Watkins HC, Guarino C, Chau A, Lopez JL, DeLisa MP, Leifer CA, Whittaker G and Putnam D

Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice

Vaccine 34: 1252-8 Link

 

 

2015

88. Ollis AA, Chai Y, Natarajan A, Perregaux EC, Jaroentomeechai T, Guarino C, Smith J, Zhang S and DeLisa MP

Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences

Scientific Reports 5: 15237 Link

87. Ollis AA, Chai Y and DeLisa MP

GlycoSNAP: a high-throughput screening methodology for engineering designer glycosylation enzymes

Methods in Molecular Biology 1321: 37-47 Link

86. Nam KH, DeLisa MP and Ke A

Characterizing metal-dependent nucleases of CRISPR/Cas prokaryotic adaptive immunity systems

Methods in Molecular Biology 1311: 265-76 Link

85. Robinson M-P, Ke N, Lobstein J, Peterson C, Szkodny A, Mansell TJ [...] Noren CJ, Taron CH, DeLisa MP and Berkmen M

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria

Nature Communications 6: 8072 Link

84. Mizrachi D, Chen Y, Liu J, Peng H-M, Ke A, Pollack L, Turner RJ, Auchus RJ and DeLisa MP

Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy

Nature Communications 6: 6826 Link

83. Boock JT, King BC, Taw M, Conrado RJ, Siu K-H, Stark J, Walker LP, Gibson DM and DeLisa MP

Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells

Journal of Molecular Biology 427: 1451-63 Link

82. Boock JT, Waraho-Zhmayev D, Mizrachi D, and DeLisa MP

Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them

Methods in Molecular Biology 1258: 79-97 Link

81. Celik E, Ollis AA, Lasanajak Y, Fisher AC, Gur G, Smith DF and DeLisa MP

Glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions

Biotechnology Journal 10: 199-209 Link

*Featured in Commentary by Dr. Eric Shusta

2014

80. Lee H-C, Portnoff AD, Rocco MA and DeLisa MP

An engineered genetic selection for ternary protein complexes inspired by a natural three-component hitchhiker mechanism

Scientific Reports 4: 7570 Link

79. Rosenthal JA, Huang C-J, Doody AM, Leung T, Mineta K, Feng DD [...] Leifer C, DeLisa MP, Mendez S and Putnam D

Mechanistic insight into Th1-biased immune responses to recombinant subunit vaccines delivered by probiotic bacteria-derived OMVs

PLoS ONE 9: e112802 Link

78. Waraho-Zhmayev D, Meksiriporn B, Portnoff AD and DeLisa MP

Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection

Protein Engineering, Design and Selection 27: 351-8 Link

77. Ollis AA, Zhang S, Fisher AC and DeLisa MP

Engineered oligosaccharyltransferases with greatly relaxed acceptor site specificity

Nature Chemical Biology 10: 816-22 Link

76. Park M, Sun Q, Liu F, DeLisa MP and Chen W

Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions

PLoS ONE 9: e97103 Link

75. Baker JL, Chen L, Rosenthal JA, Putnam D and DeLisa MP

Microbial biosynthesis of designer outer membrane vesicles

Current Opinion in Biotechnology 29: 76-84 Link

74. Portnoff AD, Stephens EA, Varner JD and DeLisa MP

Ubiquibodies: synthetic E3 ubiquitin ligases endowed with unnatural substrate specificity for targeted protein silencing

Journal of Biological Chemistry 289: 7844-55 Link

*Featured in SciBX

73. O’Brien SP and DeLisa MP

Split Cre recombinase effectively monitors protein-protein interactions in living bacteria

Biotechnology Journal 9: 355-61 Link

72. Rosenthal JA, Baker JL, Chen L, Putnam D and DeLisa MP

Pathogen-like particles: biomimetic vaccine carriers engineered at the nanoscale

Current Opinion in Biotechnology 28: 51–58 Link

71. Sun Q, Madan B, Tsai S-L, DeLisa MP and Chen W

Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis

Chemical Communications 50: 1423-5 Link

70. Haitjema CH, Boock JT, Natarajan A, Dominguez MA, Gardner JG, Keating DH, Withers ST and DeLisa MP

Universal genetic assay for engineering extracellular protein expression

ACS Synthetic Biology 3: 74–82 Link

*Featured on the cover

2013

69. Feiler C, Fisher AC, Marrichi MJ, Wright L, Schmidpeter PAM, Blankenfeldt W, Pavelka M and DeLisa MP

Directed evolution of Mycobacterium tuberculosis β-lactamase reveals gatekeeper residue that controls drug resistance and catalytic efficiency

PLoS ONE 8: e73123 Link

68. Mansell TJ, Guarino C and DeLisa MP

Engineered genetic selection links in vivo protein folding and stability with asparagine-linked glycosylation

Biotechnology Journal 8: 1445-51 Link

*Featured in Commentary by Dr. Danielle Tullman-Ercek

67. Rosenthal JA, Leung T, DeLisa MP and Putnam DA

Low-dose recombinant vaccine antigen delivery by engineered outer membrane vesicles

Nano LIFE 3: 1342002 Link

66. Baker J, Celik E and DeLisa MP

Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation

Trends in Biotechnology 31: 313-23 Link

65. Merritt JH, Ollis AA, Fisher AC and DeLisa MP

Glycans-by-design: engineering bacteria for the biosynthesis of complex glycans and glycoconjugates

Biotechnology and Bioengineering 110: 1550-64 Link

64. Waraho-Zhmayev D, Gkogka L, Yu TY and DeLisa MP

A microbial sensor for discovering structural probes of protein misfolding and aggregation

Prion 7: 151-6 Link

2012

63. Nam KH, Ding F, Haitjema CH, Huang Q, DeLisa MP and Ke A

Double-stranded endonuclease activity in B. halodurans clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas2 protein

Journal of Biological Chemistry 287: 35943-52 Link

*Featured on the cover

62. Nam KH, Haitjema CH, Ding F, DeLisa MP and Ke A

Cas5d protein processes pre-crRNA and assembles into a Cascade-like interference complex in subtype I-C/Dvulg CRISPR-Cas system

Structure 20: 1574-84 Link

*Featured in Commentary by Dr. Nicole LaRonde-LeBlanc and featured on the cover

61. Rocco MA, Waraho-Zhmayev D and DeLisa MP

Twin-arginine translocase mutations that suppress folding quality control and permit export of misfolded substrate proteins

Proceedings of the National Academies of Sciences U S A 109: 13392-7 Link

60. O’Brien SP and DeLisa MP

Functional reconstitution of a tunable E3-dependent sumoylation pathway in Escherichia coli

PLoS ONE 7: e38671 Link

59. Valderrama-Rincon JD, Fisher AC, Merritt JH, Fan Y-Y, Reading C, Chhiba K, Heiss C, Azadi P, Aebi M and DeLisa MP

An engineered eukaryotic glycosylation pathway in Escherichia coli

Nature Chemical Biology 8: 434-6 Link

*Recommended article on Faculty Opinions

58. Guarino C and DeLisa MP

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

Glycobiology 22: 596-601 Link

57. Karlsson AJ, Lim HK, Xu H, Rocco MA, Bratkowski MA, Ke A and DeLisa MP

Engineering antibody fitness and function using membrane-anchored display of correctly folded proteins

Journal of Molecular Biology 416: 94-107 Link

*Featured on the cover

56. Waraho D and DeLisa MP

Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection

Methods in Molecular Biology 813: 125-43 Link

55. Contreras Martinez L, Boock JT, Kostecki JS and DeLisa MP

The ribosomal exit tunnel as a target for optimizing protein expression in Escherichia coli

Biotechnology Journal 7: 354-60 Link

*Featured in Commentary by Dr. Francois Baneyx

54. Conrado RJ, Wu GC, Boock JT, Xu H, Chen SY, Lebar T [...] Hodnik V, Anderluh G, Dueber JE, Jerala R and DeLisa MP

DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency

Nucleic Acids Research 40: 1879-1889 Link

2011

53. Tsao C-Y, Wang L, Hashimoto Y, Yi H, March J, DeLisa MP, Wood TK, Valdes JJ, and Bentley WE

LuxS co-expression enhances yield of recombinant proteins in E. coli in part through post-transcriptional control of GroEL

Applied and Environmental Microbiology 77: 2141-52 Link

52. Qi M, Lei L, Gong S, Liu Q, DeLisa MP and Zhong G

Chlamydia trachomatis secretion of an immunodominant hypothetical protein (CT795) into host cell cytoplasm

Journal of Bacteriology 193: 2498-509 Link

51. Perez-Rodriguez R, Haitjema CH, Huang Q, Nam KH, Bernardis S, Ke A and DeLisa MP

Envelope stress is a trigger of CRISPR-mediated gene silencing in prokaryotes

Molecular Microbiology 79: 584-99 Link

*Featured in MicroCommentary by Dr. Tracy Raivio

50. Fisher AC, Rocco MA and DeLisa MP

Genetic selection of solubility-enhanced proteins using the twin-arginine translocation system

Methods in Molecular Biology 705: 53-67 Link

49. Fisher AC, Haitjema CH, Guarino C, Celik E, Endicott CE, Reading CA, Merritt JH, Ptak AC, Zhang S and DeLisa MP

Production of secretory and extracellular N-linked glycoproteins in Escherichia coli

Applied and Environmental Microbiology 77: 871-81 Link

2010

48. Chen D, Lei L, Flores R, DeLisa MP, Romesberg FE and Zhong G

Secretion of the chlamydial protease CPAF requires Sec-dependent pathway

Microbiology 156: 3031-40 Link

47. Celik E, Fisher AC, Guarino C, Mansell TJ and DeLisa MP

A phage display system for N-linked glycoproteins

Protein Science 19: 2006-13 Link

*Featured in In This Issue highlights

46. Mansell TJ, Linderman SW, Fisher AC and DeLisa MP

A rapid protein folding assay for the bacterial periplasm

Protein Science 19: 1079-90 Link

45. Borrero EE, Contreras Martinez L, DeLisa MP and Escobedo FA

Kinetics and reaction coordinates of the reassembly of protein fragments via forward flux sampling

Biophysics Journal 98: 1911-20 Link

44. Kostecki JS, Li H, Turner RJ and DeLisa MP

Visualizing interactions along the Escherichia coli twin-arginine translocation pathway using protein fragment complementation

PLoS ONE 5: e9225 Link

43. Chen D, Metzger S, Osterrieder N, DeLisa MP and Putnam D

Delivery of foreign antigens by engineered outer membrane vesicle vaccines

Proceedings of the National Academies of Sciences U S A 107: 3099-104 Link

*Featured in Nat Rev Drug Discov and SciBX

42. Lim H-K, Mansell TJ, Linderman SW, Fisher AC, Dyson MR and DeLisa MP

Mining mammalian genomes for folding competent proteins using Tat-dependent genetic selection in Escherichia coli

Protein Science 18: 2537-49 Link

 

41. Rocco MA, Kim J-Y, Burns A, Kostecki JS, Doody A, Wiesner U and DeLisa MP

Site-specific labeling of surface proteins on living cells using genetically encoded peptides that bind fluorescent nanoparticles

Bioconjugate Chemistry 20: 1482–89 Link

40. DeLisa MP and Conrado RJ

Synthetic metabolic pipelines

Nature Biotechnology 27: 728-9 Link

39. Waraho D and DeLisa MP

Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism

Proceedings of the National Academies of Sciences U S A 106: 3692-7 Link

38. Lee LL, Ha H, Chang Y-T and DeLisa MP

Discovery of amyloid-beta aggregation inhibitors using an engineered assay for protein folding and solubility

Protein Science 18: 277-286 Link

37. Fisher AC and DeLisa MP

Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery

Journal of Molecular Biology 385: 299-311 Link

2008

36. Panahandeh S, Moser M, Maurer C, DeLisa MP and Müller M

Following the path of a twin-arginine precursor along the TatABC translocase of Escherichia coli

Journal of Biology Chemistry 283: 33267-75 Link

35. Marrichi MJ, Camacho L, Russell DG and DeLisa MP

Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes across the inner membrane of bacteria

Journal of Biological Chemistry 283: 35223-35 Link

34. Conrado R, Varner JD and DeLisa MP

Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy

Current Opinion in Biotechnology 19: 492-9 Link

33. Fisher AC, Kim J-Y, Perez-Rodriguez R, Tullman-Ercek D, Fish W, Henderson LA and DeLisa MP

Exploration of twin-arginine translocation for the expression and purification of correctly folded proteins in Escherichia coli

Microbial Biotechnology 1: 403-15 Link

32. Fisher AC and DeLisa MP

Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control

PLoS ONE  3: e2351 Link

31. Kim J-Y, Doody A, Chen DJ, Cremona GH, Shuler ML, Putnam DA and DeLisa MP

Engineered bacterial outer membrane vesicles with enhanced functionality

Journal of Molecular Biology 380: 51-66 Link

 

30. DeLisa MP and Haugh J

First international conference on biomolecular engineering

Biotechnology Progress 24: 1 Link

29. Mansell TJ, Fisher AC and DeLisa MP

Engineering the protein folding landscape in Gram-negative bacteria

Current Protein and Peptide Science 9: 138-149 Link

28. Contreras Martinez L, Borrero EE, Escobedo F and DeLisa MP

In silico protein fragmentation reveals the importance of critical nuclei in domain reassembly

Biophysics Journal 94: 1575-88 Link

2007

27. Contreras Martinez L and DeLisa MP

Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability

Journal of Molecular Biology 372: 513-24 Link

26. Conrado RJ, Mansell TJ, Varner JD and DeLisa MP

Stochastic reaction-diffusion simulation of enzyme compartmentalization reveals improved catalytic efficiency for a synthetic metabolic pathway

Metabolic Engineering 9: 355-63 Link

*Featured on the cover

25. Liao Q, Subramanian G, DeLisa MP, Koch DL and Wu M

Pair velocity correlations among swimming bacteria are determined by force-quadrupole hydrodynamic interactions

Physics of Fluids 19: 061701 Link

24. Perez-Rodriguez R, Fisher AC, Perlmutter JD, Hicks MG, Chanal A, Santini C-L, Wu L-F, Palmer T and DeLisa MP

An essential role for the DnaK molecular chaperone in stabilizing over expressed substrates of the bacterial twin-arginine translocation pathway

Journal of Molecular Biology 367: 715-30 Link

23. Tullman-Ercek D, DeLisa MP, Kawarasaki Y, Iranpour P, Ribnicky B, Palmer T and Georgiou G

Export pathway selectivity of Escherichia coli twin-arginine translocation signal peptides

Journal of Biology Chemistry 282: 8309-16 Link

2006

22. Wu M, Roberts JW, Kim S, Koch DL and DeLisa MP

Collective bacterial dynamics revealed using a three-dimensional population-scale defocused particle tracking technique

Applied and Environmental Microbiology 72: 4987-94 Link

21. Diao J, Young L, Kim S, Fogarty EA, Heilman SM, Zhou P, Shuler ML, Wu M and DeLisa MP

A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis

Lab on a Chip 6: 381-388 Link

20. Contreras Martinez L, Martinez-Veracoechea F, Pohkarel P, Stroock AD, Escobedo F and DeLisa MP

Protein translocation through a tunnel induces changes in folding kinetics: a lattice model study

Biotechnology and Bioengineering 94: 105-117 Link

19. Fisher AC, Kim W and DeLisa MP

Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway

Protein Science 15: 449-458 Link

*Recommended article on Faculty Opinions

2005

18. Kim J-Y, Fogarty EA, Lu FJ, Zhu H, Wheelock GD, Henderson LA and DeLisa MP

Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli

Applied and Environmental Microbiology 71: 8451-8459 Link

17. Bronstein PA, Marrichi MJ, Cartinhour S, Schneider DJ and DeLisa MP

Identification of a twin-arginine translocation system in Pseudomonas syringe pv. tomato DC3000 and its contribution to pathogenicity and fitness

Journal of Bacteriology 187: 8450-8461 Link

2004

16. Bronstein PA, Marrichi MJ and DeLisa MP

Dissecting the twin-arginine translocation pathway using genome-wide analysis

Research in Microbiology 155: 803-810 Link

15. Fisher AC and DeLisa MP

A little help from my friends: quality control of presecretory proteins in bacteria

Journal of Bacteriology 186: 7467-7473 Link

14. Masip L, Pan J, Haldar S, Penner-Han JE, DeLisa MP, Georgiou G, Bardwell J and Collet J-F

An engineered pathway for the formation of protein disulfide bonds

Science 303: 1185-1189 Link

*Recommended article on F1000Prime

13. DeLisa MP, Lee P, Palmer T and Georgiou G

Phage shock protein PspA of Escherichia coli relieves the saturation of protein export via the Tat pathway

Journal of Bacteriology 186: 366-373 Link

*Recommended article on F1000Prime

1999-2003

12. DeLisa MP, Tullman D and Georgiou G

Folding quality control in the export of proteins via the bacterial twin-arginine translocation pathway

Proceedings of the National Academies of Sciences U S A 100: 6115-6120 Link

*Recommended article on F1000Prime

11. DeLisa MP, Samuelson P, Palmer T and Georgiou G

Genetic analysis of the twin-arginine translocator pathway in bacteria

Journal of Biological Chemistry 277: 29825-29831 Link

10. DeLisa MP and Bentley WE

Bacterial autoinduction: looking outside the cell for new metabolic engineering targets

Microbial Cell Factories 1: 5 Link

9. DeLisa MP, Valdes JJ and Bentley WE

Quorum signaling via AI-2 communicates the "metabolic burden" associated with heterologous protein production in Escherichia coli

Biotechnology and Bioengineering 75: 439-450 Link

8. DeLisa MP, Wu C-F, Wang L, Valdes JJ and Bentely WE

DNA microarray-based identification of genes controlled by AI-2 stimulated quorum sensing in Escherichia coli

Journal of Bacteriology 183: 5239-5247 Link

7. DeLisa MP, Valdes JJ and Bentley WE

Mapping stress-induced changes in autoinducer AI-2 production in chemostat-cultivated Escherichia coli

Journal of Bacteriology 183: 2918-2928 Link

6. Gill RT, DeLisa MP, Valdes JJ and Bentley WE

Genomic analysis of high cell density recombinant Escherichia coli fermentation and cell “conditioning” for improved recombinant protein yield

Biotechnology and Bioengineering 72: 85-95 Link

5. DeLisa MP, Chae HJ, Weigand WA, Rao G, Valdes JJ and Bentley WE

Generic model control of induced protein expression in high cell density cultivation of Escherichia coli using on-line GFP-fusion monitoring

Bioprocess and Biosystems Engineering 24: 83-91 Link

 

4. DeLisa MP, Zhang Z, Shiloach M, Pilevar S, Davis CC, Sirkis S and Bentley WE

Evanescent wave long period fiber Bragg grating as an immobilized antibody biosensor

Analytical Chemistry 72: 2895-2900 Link

3. Gill RT, DeLisa MP, Shiloach M, Holoman TR and Bentley WE

OmpT activity and expression increase in response to recombinant chloramphenicol acetyl-transferase overexpression

Journal of Molecular Microbiology and Biotechnology 2: 283-289 Link

2. Chae HJ, DeLisa MP, Cha HJ, Weigand WA and Bentley WE

Framework for on-line optimization of recombinant protein expression in high cell density Escherichia coli cultures using GFP-fusion monitoring

Biotechnology and Bioengineering 69: 275-285 Link
 

1. DeLisa MP, Li J, Rao G, Weigand WA and Bentley WE

Monitoring fusion protein expression during high cell density cultivation of Escherichia coli using on-line green fluorescent protein sensor

Biotechnology and Bioengineering 65: 54-64 Link

Books and Book Chapters

5. Shuler ML, Kargi F and DeLisa MP Bioprocess Engineering Third Edition. New Jersey: Prentice Hall

4. Fisher AC, Mansell TJ and DeLisa MP Protein folding and solubility: pathways and high-throughput assays In: Lutz S and Bornscheuer U, editors. Protein Engineering Handbook. Germany: John Wiley and Sons, Inc.

3. Contreras Martinez L and DeLisa MP Expression engineering of synthetic antibodies using ribosome display In: Dyson M and Durocher Y, editors. Expression Systems - Methods Express. Oxfordshire, UK: Scion Publishing

2. Conrado RJ, Mansell TJ and DeLisa MP Engineering multifunctional enzyme systems for optimized metabolite transfer between sequential conversion steps In: Smolke CD, editor. Handbook for Metabolic Pathway Engineering. San Diego: CRC Press

1. DeLisa MP, Gill RT and Bentley WE Mapping stresses in Escherichia coli to improve yield: Examining global gene regulation and “cell conditioning” strategies In: Merten O-W, editor. Recombinant Protein Production with Prokaryotic and Eukaryotic Cells A Comparative View on Host Physiology: Springer

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